000101150 001__ 101150
000101150 005__ 20180127203734.0
000101150 037__ $$aCONF
000101150 245__ $$aTandem Mass Spectrometry Protein Identification on a PC Grid
000101150 269__ $$a2007
000101150 260__ $$aNetherlands$$bIOS Press$$c2007
000101150 336__ $$aConference Papers
000101150 490__ $$aStudies in HealthTechnology and Informatics
000101150 520__ $$aWe present a method to grid-enable tandem mass spectrometry protein identification. The implemented parallelization strategy embeds the open-source x!tandem tool in a grid-enabled workflow. This allows rapid analysis of largescale mass spectrometry experiments on existing heterogeneous hardware. We have explored different data-splitting schemes, considering both splitting spectra datasets and protein databases, and examine the impact of the different schemes on scoring and computation time. While resulting peptide e-values exhibit fluctuation, we show that these variations are small, caused by statistical rather than numerical instability, and are not specific to the grid environment. The correlation coefficient of results obtained on a standalone machine versus the grid environment is found to be better than 0.933 for spectra and 0.984 for protein identification, demonstrating the validity of our approach. Finally, we examine the effect of different splitting schemes of spectra and protein data on CPU time and overall wall clock time, revealing that judicious splitting of both data sets yields best overall performance.
000101150 6531_ $$aProteomics
000101150 6531_ $$aTandem Mass Spectrometry
000101150 6531_ $$aMS/MS
000101150 6531_ $$aProtein Identification
000101150 6531_ $$aPC Grid
000101150 700__ $$0242934$$aZosso, Dominique$$g148664
000101150 700__ $$aPodvinec, Michael
000101150 700__ $$aMüller, Markus
000101150 700__ $$aAebersold, Ruedi
000101150 700__ $$aPeitsch, Manuel C.
000101150 700__ $$aSchwede, Torsten
000101150 7112_ $$a5th HealthGrid Conference$$cGeneva, Switzerland$$d22.-26.4.2007
000101150 773__ $$j126$$q3-12$$tFrom Genes to Personalized HealthCare: Grid Solutions for the Life Sciences - Proceedings of HealthGrid 2007
000101150 8564_ $$uhttp://geneva2007.healthgrid.org/$$zURL
000101150 8564_ $$iINTERNAL$$uhttps://infoscience.epfl.ch/record/101150/files/zosso_healthgrid07.pdf$$xPUBLIC
000101150 909C0 $$0252397$$pLTS
000101150 909CO $$ooai:infoscience.tind.io:101150$$pconf
000101150 917Z8 $$x148664
000101150 937__ $$aLTS-CONF-2007-015
000101150 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000101150 980__ $$aCONF