000101123 001__ 101123
000101123 005__ 20190616045611.0
000101123 0247_ $$2doi$$a10.1093/nar/gkl427
000101123 022__ $$a0305-1048
000101123 02470 $$2ISI$$a000239907800004
000101123 02470 $$2DAR$$a9148
000101123 037__ $$aARTICLE
000101123 245__ $$aShort, synthetic and selectively 13C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes
000101123 269__ $$a2006
000101123 260__ $$bOxford University Press$$c2006
000101123 336__ $$aJournal Articles
000101123 500__ $$aNational Licences
000101123 520__ $$aWe report an optimized synthesis of all canonical 2'-O-TOM protected ribonucleoside phosphoramidites and solid supports containing [13C5]-labeled ribose moieties, their sequence-specific introduction into very short RNA sequences and their use for the structure determination of two protein–RNA complexes. These specifically labeled sequences facilitate RNA resonance assignments and are essential to assign a high number of sugar–sugar and intermolecular NOEs, which ultimately improve the precision and accuracy of the resulting structures. This labeling strategy is particularly useful for the study of protein–RNA complexes with single-stranded RNA in solution, which is rapidly an increasingly relevant research area in biology.
000101123 700__ $$0240934$$g148139$$aWenter, Philipp
000101123 700__ $$0240935$$g155775$$aReymond, Luc
000101123 700__ $$aAuweter, Sigrid D.
000101123 700__ $$g172660$$aAllain, Frédéric H.-T.$$0245088
000101123 700__ $$aPitsch, Stefan$$g145837$$0240732
000101123 773__ $$j34$$tNucleic Acids Research$$k11$$qe79/1-e79/8
000101123 8564_ $$uhttps://infoscience.epfl.ch/record/101123/files/gkl427.pdf$$zPUBLISHER'S VERSION$$s4175687
000101123 909C0 $$0252378$$pLCAN
000101123 909CO $$particle$$ooai:infoscience.tind.io:101123
000101123 937__ $$aLCAN-ARTICLE-2007-002
000101123 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000101123 980__ $$aARTICLE