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  4. High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light
 
conference paper

High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light

Ivanov, Denis
•
Shcheslavskiy, Vladislav
•
Märki, Iwan  
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2009
Proc. SPIE
BIOS

We present the results on two-photon total-internal-reflection fluorescence correlation spectroscopy. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO4 laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.

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Type
conference paper
DOI
10.1117/12.807596
Web of Science ID

WOS:000285712600009

Author(s)
Ivanov, Denis
Shcheslavskiy, Vladislav
Märki, Iwan  
Leutenegger, Marcel  
Lasser, Theo  
Date Issued

2009

Published in
Proc. SPIE
Volume

7185

Issue

71850D

Subjects

Fluorescence correlation spectroscopy

•

two-photon microscopy

•

total internal reflection

•

ultrafast laser

•

radial polarisation

•

Microscopy

•

Excitation

Editorial or Peer reviewed

NON-REVIEWED

Written at

EPFL

EPFL units
LOB  
Event nameEvent placeEvent date
BIOS

San Jose, CA, USA

January 24, 2009

Available on Infoscience
March 13, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/35966
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