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  4. Second harmonic phase microscopy of collagen fibers
 
conference paper

Second harmonic phase microscopy of collagen fibers

Shaffer, Etienne  
•
Marquet, Pierre
•
Depeursinge, Christian  
2011
Multiphoton Microscopy in the Biomedical Sciences XI
Biomedical Optics (BiOS)

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional information, inaccessible to incoherent imaging schemes. Last year, we have reported how the SHG phase makes possible real-time nanometric 3D-tracking of SHG emitters, such as nanoparticles (Shaffer et al., Opt. Express 18, p.17392-17403, 2010). Here, we investigate the phase of second harmonic generated by a label-free biological specimen-more precisely collagen fibers forming the connective tissue of a mouse dermis- and discuss of its interpretation. Notably, we show how the SHG phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent SHG scattering.

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Type
conference paper
DOI
10.1117/12.874538
Web of Science ID

WOS:000297672500006

Author(s)
Shaffer, Etienne  
Marquet, Pierre
Depeursinge, Christian  
Date Issued

2011

Publisher

SPIE

Published in
Multiphoton Microscopy in the Biomedical Sciences XI
Series title/Series vol.

Proceedings of SPIE

Volume

7903

Start page

79030G

Subjects

[MVD]

URL

URL

http://spiedigitallibrary.org/proceedings/resource/2/psisdg/7903/1/79030G_1
Editorial or Peer reviewed

NON-REVIEWED

Written at

EPFL

EPFL units
LNDC  
LOA  
Event nameEvent placeEvent date
Biomedical Optics (BiOS)

San Fransisco, CA

January, 22-28, 2011

Available on Infoscience
February 20, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/64620
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