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  4. FPGA implementation of sequence-to-sequence encoder-decoder deep learning model for real-time fluorescence parameter estimation through SwissSPAD2 camera
 
conference poster

FPGA implementation of sequence-to-sequence encoder-decoder deep learning model for real-time fluorescence parameter estimation through SwissSPAD2 camera

Erbaş, İsmail
•
Mos, Paul  
•
Pandey, Vikas
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Intes, Xavier
•
Ochoa, Marien
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March 21, 2025
Multimodal Biomedical Imaging XX
Multimodal Biomedical Imaging XX

Fluorescence Lifetime Imaging (FLI) is widely used in biomedical research for its high sensitivity and detailed cellular insights. It is effective for multiplexed studies and provides information about metabolic states, protein interactions, and ligand-target engagements, independent of signal intensity and fluorophore concentration. Traditional FLI techniques are slow and computationally demanding. This study addresses these limitations by implementing a deep learning-based sequence-to-sequence model directly into the SwissSPAD2 (SS2), a photon-counting camera coupled to an FPGA. The integration, enhanced by efficient resource management and parallel computation, allows for near-real-time output of FLI parameters. We tested the performance using in-silico and in-vitro data, demonstrating that this approach advances the feasibility of real-time FLI analysis, with promising implications for both preclinical and clinical applications.

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Type
conference poster
DOI
10.1117/12.3049435
Author(s)
Erbaş, İsmail

Rensselaer Polytechnic Institute

Mos, Paul  

EPFL

Pandey, Vikas

Rensselaer Polytechnic Institute

Bruschini, Claudio  

EPFL

Charbon, Edoardo  

EPFL

Intes, Xavier

Rensselaer Polytechnic Institute

Editors
Intes, Xavier
•
Ochoa, Marien
•
Yaseen, Mohammad A.
Date Issued

2025-03-21

Publisher

SPIE

Published in
Multimodal Biomedical Imaging XX
Series title/Series vol.

Proceedings SPIE; PC13309

Start page

41

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
AQUA  
Event nameEvent acronymEvent placeEvent date
Multimodal Biomedical Imaging XX

San Francisco, United States

2025-01-25 - 2025-01-31

Available on Infoscience
December 23, 2025
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/257285
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