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research article

Compact, high-speed multi-directional selective plane illumination microscopy

Power, Rory M.
•
Schlaeppi, Anjalie  
•
Huisken, Jan
April 1, 2023
Biomedical Optics Express

We present an elegant scheme for providing multi-directional illumination in selective plane illumination microscopy (SPIM). Light sheets can be delivered from one of two opposed directions at a time and pivoted about their center for efficient stripe artifact suppression using only a single galvanometric scanning mirror to perform both functions. The scheme results in a much smaller instrument footprint and allows multi-directional illumination with reduced expense compared with comparable schemes. Switching between the illumination paths is near instantaneous and the whole-plane illumination scheme of SPIM maintains the lowest rates of photodamage, which is often sacrificed by other recently reported destriping strategies. The ease of synchronization allows this scheme to be used at higher speeds than resonant mirrors typically used in this regard. We provide validation of this approach in the dynamic environment of the zebrafish beating heart, where imaging at up to 800 frames per second is demonstrated alongside efficient suppression of artifacts. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

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Type
research article
DOI
10.1364/BOE.476217
Web of Science ID

WOS:000970419400003

Author(s)
Power, Rory M.
Schlaeppi, Anjalie  
Huisken, Jan
Date Issued

2023-04-01

Published in
Biomedical Optics Express
Volume

14

Issue

4

Start page

1445

End page

1459

Subjects

Biochemical Research Methods

•

Optics

•

Radiology, Nuclear Medicine & Medical Imaging

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Biochemistry & Molecular Biology

•

Optics

•

Radiology, Nuclear Medicine & Medical Imaging

•

light

•

reconstruction

•

dynamics

•

deep

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

Available on Infoscience
May 8, 2023
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/197471
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