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  4. Development of an Array of Ion-Selective Microelectrodes Aimed for the Monitoring of Extracellular Ionic Activities
 
research article

Development of an Array of Ion-Selective Microelectrodes Aimed for the Monitoring of Extracellular Ionic Activities

Guenat, O.
•
Generelli, S.
•
de Rooij, N. F.  
Show more
2006
Analytical Chemistry

In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4 +) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or differentiation. The platform for cell culture is equipped with an array of 16 silicon nitride micropipet-based ion-selective microelectrodes with a diameter of either 2 or 6 μm. This array is located at the bottom of a 200-μm-wide and 350-μm-deep microwell where the cells are cultured. The characterization of the ion-selective microelectrode arrays in different standard and physiological solutions is presented. Near-Nernstian slopes were obtained for potassium- (58.6 ± 0.8 mV/pK, n = 15) and ammonium-selective microelectrodes (59.4 ± 3.9 mV/pNH4, n = 13). The calibration curves were highly reproducible and showed an average drift of 4.4 ± 2.3 mV/h (n = 10). Long-term behavior and response after immersion in physiological solutions are also presented. The lifetime of the sensors was found to be extremely long with a high recovery rate. © 2006 American Chemical Society.

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Type
research article
DOI
10.1021/ac0609733
Author(s)
Guenat, O.
•
Generelli, S.
•
de Rooij, N. F.  
•
Koudelka-Hep, M.
•
Berthiaume, F.
•
Yarmusch, M. L.
Date Issued

2006

Published in
Analytical Chemistry
Volume

78

Issue

21

Start page

7453

End page

7460

Note

386

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
SAMLAB  
Available on Infoscience
May 12, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/39335
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