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  4. Hierarchy of the Components in Spray-Dried, Protein-Excipient Particles Using DNP-Enhanced NMR Spectroscopy
 
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research article

Hierarchy of the Components in Spray-Dried, Protein-Excipient Particles Using DNP-Enhanced NMR Spectroscopy

Berruyer, Pierrick  
•
Lindkvist, Maria
•
Gracin, Sandra
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October 2, 2023
Molecular Pharmaceutics

Protein-based drugs are becoming increasingly important, but there are challenges associated with their formulation (for example, formulating stable inhalable aerosols while maintaining the proper long-term stability of the protein). Determining the morphology of multicomponent, protein-based drug formulations is particularly challenging. Here, we use dynamic nuclear polarization (DNP) solid-state NMR spectroscopy to determine the hierarchy of components within spray-dried particles containing protein, trehalose, leucine, and trileucine. DNP NMR was applied to these formulations to assess the localization of the components within the particles. We found a consistent scheme, where trehalose and the protein are co-located within the same phase in the core of the particles and leucine and trileucine are distributed in separate phases at the surface of the particles. The description of the hierarchy of the organic components determined by DNP NMR enables the rationalization of the performance of the formulation.

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Type
research article
DOI
10.1021/acs.molpharmaceut.3c00539
Web of Science ID

WOS:001079197600001

Author(s)
Berruyer, Pierrick  
•
Lindkvist, Maria
•
Gracin, Sandra
•
Starciuc, Tatiana
•
Bertarello, Andrea  
•
Busi, Baptiste  
•
Schantz, Staffan
•
Emsley, Lyndon  
Date Issued

2023-10-02

Publisher

Amer Chemical Soc

Published in
Molecular Pharmaceutics
Volume

20

Issue

11

Start page

5682

End page

5689

Subjects

Life Sciences & Biomedicine

•

Nmr Spectroscopy

•

Dynamic Nuclear Polarization

•

Protein Drug Formulations

•

Structure

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LRM  
Available on Infoscience
February 14, 2024
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/203720
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