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  4. The studies of ParA and ParB dynamics reveal asymmetry of chromosome segregation in mycobacteria
 
research article

The studies of ParA and ParB dynamics reveal asymmetry of chromosome segregation in mycobacteria

Ginda, Katarzyna
•
Santi, Isabella  
•
Bousbaine, Djenet
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2017
Molecular Microbiology

Active segregation of bacterial chromosomes usually involves the action of ParB proteins, which bind in proximity of chromosomal origin (oriC) regions forming nucleoprotein complexes - segrosomes. Newly duplicated segrosomes are moved either uni- or bidirectionally by the action of ATPases - ParA proteins. In Mycobacterium smegmatis the oriC region is located in an off-centred position and newly replicated segrosomes are segregated towards cell poles. The elimination of M. smegmatis ParA and/or ParB leads to chromosome segregation defects. Here, we took advantage of microfluidic time-lapse fluorescent microscopy to address the question of ParA and ParB dynamics in M. smegmatis and M. tuberculosis cells. Our results reveal that ParB complexes are segregated in an asymmetrical manner. The rapid movement of segrosomes is dependent on ParA that is transiently associated with the new pole. Remarkably in M. tuberculosis, the movement of the ParB complex is much slower than in M. smegmatis, but segregation as in M. smegmatis lasts approximately 10% of the cell cycle, which suggests a correlation between segregation dynamics and the growth rate. On the basis of our results, we propose a model for the asymmetric action of segregation machinery that reflects unequal division and growth of mycobacterial cells.

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Type
research article
DOI
10.1111/mmi.13712
Web of Science ID

WOS:000405994500010

Author(s)
Ginda, Katarzyna
•
Santi, Isabella  
•
Bousbaine, Djenet
•
Zakrzewska-Czerwinska, Jolanta
•
Jakimowicz, Dagmara
•
McKinney, John D.  
Date Issued

2017

Publisher

Wiley

Published in
Molecular Microbiology
Volume

105

Issue

3

Start page

453

End page

468

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPKIN  
Available on Infoscience
September 5, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/140150
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