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research article

Low-density lipoprotein analysis in microchip capillary electrophoresis systems

Ceriotti, L.
•
Shibata, T.
•
Folmer, B.
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2002
Electrophoresis

Due to the mounting evidence for altered lipoprotein and cholesterol-lipoprotein content in several disease states, there has been an increasing interest in analytical methods for lipoprotein profiling for diagnosis. The separation of low- and high-density lipoproteins (LDL and HDL, respectively) has been recently demonstrated using a microchip capillary electrophoresis (CE) system [1]. In contrast to this previous study, the present report demonstrates that LDL analysis can be performed in an uncoated glass microchannel. Moreover, by adding sodium dodecyl sulfate (SDS) to the sample at a concentration well below the critical micellar concentration prior to injection, the LDL peak undergoes a focusing effect and exhibits an apparent efficiency of 2.2 × 107 plates/m. Laser light scattering experiments demonstrate that the low concentration of SDS used does not significantly alter lipoprotein particle size distribution within the time course that the analysis is performed. It is thus hypothesized that SDS nondisruptively coats LDL particles. The peak sharpening effect, observed only when SDS is added solely to the sample, is probably due to a mobility gradient created between the sample and the running buffer. The chip-based method demonstrated here has the potential for rapid analysis and sensitive detection of different LDL forms of clinical relevance.

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Type
research article
DOI
10.1002/1522-2683(200210)23:20<3615::AID-ELPS3615>3.0.CO;2-J
Author(s)
Ceriotti, L.
•
Shibata, T.
•
Folmer, B.
•
Weiller, B.
•
Roberts, M. A.
•
de Rooij, N. F.  
•
Verpoorte, E.
Date Issued

2002

Published in
Electrophoresis
Volume

23

Issue

20

Start page

3615

End page

3622

Note

276

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
SAMLAB  
Available on Infoscience
May 12, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/39146
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