Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. Liposome nano-formulation with cationic polar lipid DOTAP and cholesterol as a suitable pH-responsive carrier for molecular therapeutic drug (all-trans retinoic acid) delivery to lung cancer cells
 
Loading...
Thumbnail Image
research article

Liposome nano-formulation with cationic polar lipid DOTAP and cholesterol as a suitable pH-responsive carrier for molecular therapeutic drug (all-trans retinoic acid) delivery to lung cancer cells

Grace, Viswanathan Mariammal Berlin
•
Wilson, Devarajan David
•
Guruvayoorappan, Chandrasekharan
Show more
March 8, 2021
Iet Nanobiotechnology

The molecular targeted drug ATRA demands a suitable carrier that delivers to the cancer site due to its poor bioavailability and drug resistance. ATRA, being a lipid with carboxylic acid, has been nano-formulated as a cationic lipo-ATRA with DOTAP:cholesterol:ATRA (5:4:1) and its pH-responsive release, intracellular drug accumulation, and anticancer effect on human lung cancer (A549) cell line analysed. The analysis of the physicochemical characteristics of the developed lipo-ATRA (0.8 mu mol) revealed that the size of 231 +/- 2.35 d.nm had a zeta potential of 6.4 +/- 1.19 and an encapsulation efficiency of 93.7 +/- 3.6%. The ATRA release from lipo-ATRA in vitro was significantly (p <= 0.05) higher at acidic pH 6 compared to pH 7.5. The intracellular uptake of ATRA into lipo-ATRA-treated A549 cells was seven-fold higher (0.007 +/- 0.001 mg/ml) while only three-fold uptake was observed in free ATRA treatment (0.003 +/- 0.002 mg/ml). The lipo-ATRA treatment caused a highly significant (p <= 0.001) decrease in percent cell viability at 48 h when compared with the free ATRA treatment. Overall, the results proved that the developed lipo-ATRA has suitable physicochemical properties with enhanced ATRA release at acidic pH, while maintaining stability at physiologic pH and temperature. This resulted in an increased ATRA uptake by lung cancer cells with enhanced treatment efficiency. Hence, it is concluded that DOTAP lipo-ATRA is a suitable carrier for ATRA delivery to solid cancer cells.

  • Details
  • Metrics
Type
research article
DOI
10.1049/nbt2.12028
Web of Science ID

WOS:000627173300001

Author(s)
Grace, Viswanathan Mariammal Berlin
•
Wilson, Devarajan David
•
Guruvayoorappan, Chandrasekharan
•
Danisha, Jesubatham Perinba
•
Bonati, Lucia  
Date Issued

2021-03-08

Publisher

WILEY

Published in
Iet Nanobiotechnology
Volume

15

Issue

4

Start page

380

End page

390

Subjects

Biochemical Research Methods

•

Nanoscience & Nanotechnology

•

Biochemistry & Molecular Biology

•

Science & Technology - Other Topics

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBI  
Available on Infoscience
April 10, 2021
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/177140
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés