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research article

Signal strength and integrated intensity in confocal and image scanning microscopy

Sheppard, Colin J. R.
•
Castello, Marco
•
Tortarolo, Giorgio  
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January 1, 2023
Journal Of The Optical Society Of America A-Optics Image Science And Vision

The properties of signal strength and integrated intensity in a scanned imaging system are reviewed. These prop-erties are especially applied to confocal imaging systems, including image scanning microscopy. The integrated intensity, equal to the image of a uniform planar (sheet) object, rather than the peak of the point spread function, is a measure of the flux in an image. Analytic expressions are presented for the intensity in the detector plane for a uniform volume object, and for the resulting background. The variation in the integrated intensity with defocus for an offset point detector is presented. This axial fingerprint is independent of any pixel reassignment. The intensity in the detector plane is shown to contain the defocus information, and simple processing of the recorded data can improve optical sectioning and background rejection.(c) 2022 Optica Publishing Group

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Type
research article
DOI
10.1364/JOSAA.477240
Web of Science ID

WOS:000906440100006

Author(s)
Sheppard, Colin J. R.
Castello, Marco
Tortarolo, Giorgio  
Zunino, Alessandro
Slenders, Eli
Bianchini, Paolo
Vicidomini, Giuseppe
Diaspro, Alberto
Date Issued

2023-01-01

Publisher

Optica Publishing Group

Published in
Journal Of The Optical Society Of America A-Optics Image Science And Vision
Volume

40

Issue

1

Start page

138

End page

148

Subjects

Optics

•

pixel reassignment

•

super-resolution

•

optical transfer

•

detector

•

pinhole

•

noise

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LEB  
Available on Infoscience
January 30, 2023
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/194443
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