Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on microfabricated flow cytometer
Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment.
Kuttel_ActaTr07_Babesia.pdf
openaccess
327.04 KB
Adobe PDF
101a95c79b7fc0dc44ee01765970020a