Understanding the role of ecosystem services (ES) and their interactions at different spatial scales in territorial space governance is crucial for sustainable development. However, a unified analysis of scale and zoning effects on ES supporting sustainable development remains a theoretical and practical gap. Therefore, this study utilized the traditional concept of the modifiable areal unit problem (MAUP) to establish the ES-MAUP framework. Additionally, the highly urbanized region of Southern Jiangsu, China, was selected as a case study area to quantitatively evaluate four typical regulating ecosystem services in 2020: urban cooling (UC), flood mitigation (FM), soil conservation (SC), and net primary productivity (NPP). The spatial characteristic information of these four ES was effectively preserved with increasing spatial scale. Significant differences existed in the hotspot patterns of NPP, UC, and SC among different zoning units. Across the three zoning units, the correlations between NPP-UC, NPP-SC, and UC-SC exhibited consistent positive trends, while notable differences in correlations between other ES pairs, such as NPP-FM and UC-FM.