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  4. Atypical and distinct microtubule radial symmetries in the centriole and the axoneme of Lecudina tuzetae
 
research article

Atypical and distinct microtubule radial symmetries in the centriole and the axoneme of Lecudina tuzetae

Bezler, Alexandra  
•
Woglar, Alexander  
•
Schneider, Fabian  
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July 1, 2022
Molecular Biology Of The Cell

The centriole is a minute cylindrical organelle present in a wide range of eukaryotic species. Most centrioles have a signature ninefold radial symmetry of microtubules that is imparted to the axonemes of the cilia and flagella they template, with nine centriolar microtubule doublets growing into nine axonemal microtubule doublets. There are exceptions to the ninefold symmetrical arrangement of axonemal microtubules in some species, with lower or higher fold symmetries. In the few cases where this has been examined, such alterations in axonemal symmetries are grounded in similar alterations in centriolar symmetries. Here, we examine the question of microtubule number continuity between centriole and axoneme in flagellated gametes of the gregarine Lecudina tuzetae, which have been reported to exhibit a sixfold radial symmetry of axonemal microtubules. We used time-lapse differential interference microscopy to identify the stage at which flagellated gametes are present. Thereafter, using electron microscopy and ultrastructure-expansion microscopy coupled to stimulated emission depletion superresolution imaging, we uncover that a six- or fivefold radial symmetry in the axoneme is accompanied by an eightfold radial symmetry in the centriole. We conclude that the transition between centriolar and axonemal microtubules can be characterized by unexpected plasticity.

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Type
research article
DOI
10.1091/mbc.E22-04-0123
Web of Science ID

WOS:000821120800004

Author(s)
Bezler, Alexandra  
Woglar, Alexander  
Schneider, Fabian  
Douma, Friso  
Buergy, Leo  
Busso, Coralie  
Goenczy, Pierre  
Date Issued

2022-07-01

Publisher

AMER SOC CELL BIOLOGY

Published in
Molecular Biology Of The Cell
Volume

33

Issue

8

Article Number

ar75

Subjects

Cell Biology

•

Cell Biology

•

spermiogenesis

•

spermatozoon

•

architecture

•

flagellum

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sperm

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cilia

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPGON  
Available on Infoscience
July 18, 2022
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/189255
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