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research article
Fluorogenic Probes for Multicolor Imaging in Living Cells
Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag are fluorogenic, cell-permeable and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
Type
research article
Web of Science ID
WOS:000381062600008
Authors
Lukinavičius, Gražvydas
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Umezawa, Keitaro
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D’Este, Elisa
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Göttfert, Fabian
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Ta, Haisen
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Hell, Stefan W.
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Urano, Yasuteru
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Publication date
2016
Publisher
Published in
Volume
138
Issue
30
Start page
9365
End page
9368
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
July 27, 2016
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