Transfer and disproportionation reaction of the cyclodextrin glucanotransferase from Klebsiella pneumoniae
Cyclodextrin glucanotransferases not only transform starch into cyclodextrins, but also catalyze the transfer of cyclodextrins to appropriate acceptors and the disproportionation of linear dextrins. These three reactions are manifestations of a single catalytic activity. The transfer of a-cyclodextrin to maltitol as well as the disproportionation of functionalized dextrins carrying maltitol at the reducing end are studied in the presence of the cyclodextrin glucanotransferase of Klebsiella pneumoniae. The transfer reaction is a fast process followed by the disproportionation of the primary product. The primary functionalized dextrin is degraded in the presence of excess acceptor mostly by the exchange of maltose units. Studies on the disproportionation of purified functionalized linear dextrins reveals that the acceptor is the most prominent product. Apparently, disproportionation leads to the synthesis of starch. [on SciFinder (R)]
1996
45
50
CAN 126:185073
16-9
Fermentation and Bioindustrial Chemistry
Department of Technical Sciences,University of Bielefeld,Bielefeld,Germany.
Conference
written in English.
9030-09-5 (Cyclodextrin glucanotransferase) Role: BAC (Biological activity or effector, except adverse), BSU (Biological study, unclassified), BIOL (Biological study) (transfer and disproportionation reaction of cyclodextrin glucanotransferase from Klebsiella pneumoniae); 585-88-6P (Maltitol) Role: BPN (Biosynthetic preparation), BIOL (Biological study), PREP (Preparation) (transfer and disproportionation reaction of cyclodextrin glucanotransferase from Klebsiella pneumoniae); 10016-20-3 (a-Cyclodextrin) Role: BPR (Biological process), BSU (Biological study, unclassified), BIOL (Biological study), PROC (Process) (transfer and disproportionation reaction of cyclodextrin glucanotransferase from Klebsiella pneumoniae); 9004-53-9 (Dextrin) Role: BSU (Biological study, unclassified), BIOL (Biological study) (transfer and disproportionation reaction of cyclodextrin glucanotransferase from Klebsiella pneumoniae)
EPFL