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  4. Detection of TERRA R-Loops at Human Telomeres
 
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Detection of TERRA R-Loops at Human Telomeres

Glousker, Galina  
•
Valador Fernandes, Rita  
•
Feretzaki, Marianna  
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Aguilera, Andrés
•
Ruzov, Alexey
2022
R-Loops . Methods in Molecular Biology

R-loops are three-stranded nucleic acid structures composed of a DNA–RNA hybrid and a displaced DNA strand. The long noncoding RNA TERRA forms R-loops at telomeres influencing the telomeric chromatin composition and impacting on telomere maintenance mechanisms by semiconservative DNA replication, homology directed DNA repair and telomerase. Here, we describe a method to detect R-loops at telomeres, which involves immunoprecipitation with the R-loop recognizing S9.6 antibody, followed by detection of telomeric DNA by either dot-blot hybridization with a radiolabeled telomeric probe, or qPCR using DNA primers that are specific for subtelomeric sequences.

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Type
book part or chapter
DOI
10.1007/978-1-0716-2477-7_11
Author(s)
Glousker, Galina  
•
Valador Fernandes, Rita  
•
Feretzaki, Marianna  
•
Lingner, Joachim  
Editors
Aguilera, Andrés
•
Ruzov, Alexey
Date Issued

2022

Publisher

Springer US

Publisher place

New York

Published in
R-Loops . Methods in Molecular Biology
DOI of the book
10.1007/978-1-0716-2477-7
ISBN of the book

978-1-0716-2476-0

Total of pages

468

Start page

159

End page

171

Series title/Series vol.

MIMB Methods in Molecular Biology; 2528

ISSN (of the series)

1064-3745

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPLIN  
Available on Infoscience
August 13, 2024
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/240706
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