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Aptamer-field-effect transistors for small-molecule sensing in complex environments

Nakatsuka, Nako  
Mayer, Günter
•
Menger, Marcus M.
2022
Nucleic Acid Aptamers: Selection, Characterization, and Application

Aptamer-functionalized field-effect transistor (FET) biosensors enable detection of small-molecule targets in complex environments such as tissue and blood. Conventional FET-based platforms suffer from Debye screening in high ionic strength physiological environments where the effective sensing distance is limited to less than a nanometer from the surface of the sensor. Aptamers that undergo significant conformational rearrangement of negatively charged backbones upon target recognition within or in close proximity to the Debye length, facilitate the transduction of electronic signals through the semiconducting channel. Herein, the fabrication of high-performance, ultrathin-film FETs and subsequent aptamer functionalization are described. Moreover, electronic sensing measurement protocols alongside calibration methods to minimize device-to-device variations are covered.

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Type
book part or chapter
DOI
10.1007/978-1-0716-2695-5_14
Author(s)
Nakatsuka, Nako  
Editors
Mayer, Günter
•
Menger, Marcus M.
Date Issued

2022

Publisher

Humana

Publisher place

New York, NY

Published in
Nucleic Acid Aptamers: Selection, Characterization, and Application
ISBN of the book

978-1-0716-2694-8

Start page

187

End page

196

Series title/Series vol.

Methods in Molecular Biology (MIMB); 2570

Subjects

Biosensor

•

Device fabrication

•

DNA aptamer

•

Field-effect transistor

•

Protocol

Written at

OTHER

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CHEMINA  
RelationURL/DOI

IsSupplementedBy

https://doi.org/10.1007/978-1-0716-2695-5_14
Available on Infoscience
January 23, 2024
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/203093
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