Fractional charges are one of the wonders of the fractional quantum Hall effect. Such objects are also anticipated in two-dimensional hexagonal lattices under time reversal symmetry-emerging as bound states of a rotating bond texture called a Kekule vortex. However, the physical mechanisms inducing such topological defects remain elusive, preventing experimental realization. Here, we report the observation of Kekule vortices in the local density of states of graphene under time reversal symmetry. The vortices result from intervalley scattering on chemisorbed hydrogen adatoms. We uncover that their 2 pi winding is reminiscent of the Berry phase pi of the massless Dirac electrons. We can also induce a Kekule pattern without vortices by creating point scatterers such as divacancies, which break different point symmetries. Our local-probe study thus confirms point defects as versatile building blocks for Kekule engineering of graphene's electronic structure.|Kekule vortices in hexagonal lattices can host fractionalized charges at zero magnetic field, but have remained out of experimental reach. Here, the authors report a Kekule vortex in the local density states of graphene around a chemisorbed hydrogen adatom.