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  4. Two-photon excitation endoscopy through a multimode optical fiber
 
conference paper

Two-photon excitation endoscopy through a multimode optical fiber

Delgado, Edgar E. Morales
•
Psaltis, Demetri
•
Moser, Christophe  
Bifano, Tg
•
Kubby, J
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2016
Adaptive Optics And Wavefront Control For Biological Systems Ii
Conference on Adaptive Optics and Wavefront Control for Biological Systems II

The vast number of propagating solutions to the wave equation in multimode optical fibers represents a larger information capacity than provided by fiber bundles of the same diameter. Therefore, in the field of imaging, multimode fibers potentially allow the transmission of images with higher resolution. However, image transmission through multimode fibers is not direct as in the fiber bundle case, in which each of the fiber cores can relay a portion of the distal image. In multimode fiber transmission, a distribution of intensity is scrambled in time and space by the propagating modes, leading to a speckle-like pattern that does not resemble the initial distribution. Here, we demonstrate two-photon excitation imaging of fluorescent beads through a multimode optical fiber. We show that our method maintains the advantages of two-photon excitation microscopy compared to single-photon excitation such as reduced photo-bleaching, deeper penetration depth and sectioning capability. Our method is based on time-gated digital phase conjugation, which allows the generation of focused pulses on the other side of a multimode fiber. To acquire an image, the focused femtosecond pulse is scanned in a three-dimensional mesh, producing two-photon excitation on each spatial location of the sample. By collecting the fluorescence through the fiber, a 3D two-photon image is reconstructed.

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Type
conference paper
DOI
10.1117/12.2209604
Web of Science ID

WOS:000389085900041

Author(s)
Delgado, Edgar E. Morales
Psaltis, Demetri
Moser, Christophe  
Editors
Bifano, Tg
•
Kubby, J
•
Gigan, S
Date Issued

2016

Publisher

Spie-Int Soc Optical Engineering

Publisher place

Bellingham

Published in
Adaptive Optics And Wavefront Control For Biological Systems Ii
ISBN of the book

978-1-62841-951-1

Total of pages

4

Series title/Series vol.

Proceedings of SPIE

Volume

9717

Start page

97171E

Subjects

Fiber optics imaging

•

Endoscopic imaging

•

Phase conjugation

•

Femtosecond phenomena

•

Multiphoton imaging

•

Nonlinear microscopy

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LC  
LAPD  
Event nameEvent placeEvent date
Conference on Adaptive Optics and Wavefront Control for Biological Systems II

San Francisco, CA

FEB 13-15, 2016

Available on Infoscience
January 24, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/133349
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