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  4. Genistein increases the thermogenic program of subcutaneous WAT and increases energy expenditure in mice
 
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research article

Genistein increases the thermogenic program of subcutaneous WAT and increases energy expenditure in mice

Palacios-Gonzalez, Berenice
•
Vargas-Castillo, Ariana
•
Alejandra Velazquez-Villegas, Laura  
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June 1, 2019
Journal Of Nutritional Biochemistry

White adipose tissue (WAT) can differentiate into beige adipose tissue by the browning process. Some polyphenols, including isoflavones, particularly genistein, are suggested to increase the expression of browning markers. There is evidence that consumption of genistein can attenuate body weight gain and improve glucose tolerance and blood lipid levels. The aim of the present study was to investigate the potential mechanisms of stimulation by which genistein activates the browning of WAT. We studied the stimulation of the expression of browning markers in the following models: mice fed genistein; preadipocytes from 3 T3-L1 cells; and the stromal vascular fraction (SVF) from the inguinal adipose tissue of mice. The results indicated that genistein can stimulate the browning process by at least two mechanisms. An indirect mechanism was involved in the induction of PGC-1 alpha/FNDC5 in skeletal muscle leading to an increase in the myokine irisin. In preadipocytes, irisin was able to increase the expression of Ucpl and Tmem26, markers of browning, to increase energy expenditure. Interestingly, genistein was also able to activate browning by a direct mechanism. Incubation of preadipocytes with genistein increased UCP1 expression as well as some biomarkers of browning in a concentration-dependent manner, possibly via phosphorylation of AMPK. The effect of genistein was accompanied by an increase in the number of mitochondria as well as in the maximum respiration rate of the adipocytes. In conclusion, this study indicated that genistein can increase energy expenditure by stimulating the browning process directly in preadipocytes and indirectly by increasing the circulating levels of irisin. (C) 2019 Elsevier Inc. All rights reserved.

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Type
research article
DOI
10.1016/j.jnutbio.2019.03.012
Web of Science ID

WOS:000471736800007

Author(s)
Palacios-Gonzalez, Berenice
•
Vargas-Castillo, Ariana
•
Alejandra Velazquez-Villegas, Laura  
•
Vasquez-Reyes, Sarai
•
Lopez, Patricia
•
Noriega, Lilia G.  
•
Aleman, Gabriela
•
Tovar-Palacio, Claudia
•
Torre-Villalvazo, Ivan
•
Yang, Li-Jun
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Date Issued

2019-06-01

Publisher

ELSEVIER SCIENCE INC

Published in
Journal Of Nutritional Biochemistry
Volume

68

Start page

59

End page

68

Subjects

Biochemistry & Molecular Biology

•

Nutrition & Dietetics

•

adipose tissue

•

energy expenditure

•

genistein

•

irisin

•

polyphenols

•

skeletal muscle

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white adipose-tissue

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insulin-resistance

•

messenger-rna

•

body-weight

•

fat

•

brown

•

beige

•

adipocytes

•

cell

•

lipotoxicity

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPSCHOONJANS  
LISP  
Available on Infoscience
June 28, 2019
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/158624
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