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review article

Fluorescence microscopy: A statistics-optics perspective

Fazel, Mohamadreza
•
Grussmayer, Kristin S.
•
Ferdman, Boris
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June 5, 2024
Reviews Of Modern Physics

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scales on par with or smaller than light's emission wavelength. Here the optics responsible for generating fluorescent images, fluorophore properties, and microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement, are reviewed.

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Type
review article
DOI
10.1103/RevModPhys.96.025003
Web of Science ID

WOS:001241660900001

Author(s)
Fazel, Mohamadreza
•
Grussmayer, Kristin S.
•
Ferdman, Boris
•
Radenovic, Aleksandra  
•
Shechtman, Yoav
•
Berrie, Russel
•
Enderlein, Jaerg
•
Presse, Steve
Date Issued

2024-06-05

Publisher

Amer Physical Soc

Published in
Reviews Of Modern Physics
Volume

96

Issue

2

Article Number

025003

Subjects

Physical Sciences

•

Structured-Illumination Microscopy

•

Point-Spread-Function

•

Light-Sheet Microscopy

•

Single-Molecule Fret

•

Multicolor Localization Microscopy

•

Patterned Excitation Microscopy

•

Protein-Protein Interactions

•

Induced Energy-Transfer

•

Correlation Spectroscopy

•

Adaptive Optics

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBEN  
FunderGrant Number

NIH NIGMS

R01GM130745

NIH MIRA

R35GM148237

European Research Council (ERC)

802567

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Available on Infoscience
July 3, 2024
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/208996
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