conference paper
Microfluidic Device for Nucleic Acid Fragmentation by Shear Force
2002
Micro Total Analysis Systems 2002
A microfluidic device for random fragmentation of DNA or RNA based on the application of a hydrodynamic shear force to these biomolecules is presented. Different from conventional fragmentation instruments, the microchip-based implementation allows molecules to be repetitively sheared and broken during a single run through the device. It therefore requires comparatively few external components and has a low dead volume in the order of 0.5 μL. 48 kbp λDNA could be fragmented down to an average size of 4 kbp at a flow rate of 1 mL/min.
Type
conference paper
Author(s)
Editors
Baba, Yoshinobu
•
Shoji, Shuichi
•
vad den Berg, Albert
Date Issued
2002
Publisher
Publisher place
Berlin
Published in
Micro Total Analysis Systems 2002
Volume
1
Start page
172
End page
175
Subjects
Editorial or Peer reviewed
NON-REVIEWED
Written at
OTHER
EPFL units
| Event name | Event place | Event date |
Nara, Japan | 3–7 November 2002 | |
Available on Infoscience
May 12, 2009
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