research article
SpiroZin1: A Reversible and pH-Insensitive, Reaction-Based, Red-Fluorescent Probe for Imaging Biological Mobile Zinc
Rivera-Fuentes, Pablo
•
Lippard, Stephen J.
2014
A reversible, reaction-based sensor for biol. mobile zinc was designed, prepd., and characterized. The sensing mechanism of this probe is based on the zinc-induced, ring-opening reaction of spirobenzopyran to give a cyanine fluorophore that emits in the deep-red region of the electromagnetic spectrum. This probe is not activated by protons and operates efficiently in aq. soln. at pH 7 and high ionic strength. The mechanism of this reaction was studied by using a combination of kinetics expts. and DFT calcns. The biocompatibility of the probe was demonstrated in live HeLa cells.
Type
research article
Author(s)
Rivera-Fuentes, Pablo
Lippard, Stephen J.
Date Issued
2014
Published in
Volume
9
Start page
1238
End page
1243
Subjects
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Editorial or Peer reviewed
REVIEWED
Written at
OTHER
EPFL units
Available on Infoscience
October 30, 2019
Use this identifier to reference this record