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research article
Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.
The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
Type
research article
PubMed ID
9226276
Publication date
1997
Published in
Volume
179
Issue
14
Start page
4627
End page
4630
Peer reviewed
REVIEWED
Written at
OTHER
EPFL units
Available on Infoscience
September 7, 2010
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