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  4. NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha
 
research article

NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha

van der Voet, Monique
•
Berends, Christian W H
•
Perreault, Audrey
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2009
Nature cell biology

The spindle apparatus dictates the plane of cell cleavage, which is critical in the choice between symmetric or asymmetric division. Spindle positioning is controlled by an evolutionarily conserved pathway, which involves LIN-5/GPR-1/2/Galpha in Caenorhabditis elegans, Mud/Pins/Galpha in Drosophila and NuMA/LGN/Galpha in humans. GPR-1/2 and Galpha localize LIN-5 to the cell cortex, which engages dynein and controls the cleavage plane during early mitotic divisions in C. elegans. Here we identify ASPM-1 (abnormal spindle-like, microcephaly-associated) as a novel LIN-5 binding partner. ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle poles. Spindle rotation during maternal meiosis is independent of GPR-1/2 and Galpha, yet requires LIN-5, ASPM-1, CMD-1 and dynein. Our data support the existence of two distinct LIN-5 complexes that determine localized dynein function: LIN-5/GPR-1/2/Galpha at the cortex, and LIN-5/ASPM-1/CMD-1 at spindle poles. These functional interactions may be conserved in mammals, with implications for primary microcephaly

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Type
research article
DOI
10.1038/ncb1834
Web of Science ID

WOS:000263777900010

Author(s)
van der Voet, Monique
Berends, Christian W H
Perreault, Audrey
Nguyen-Ngoc, Tu
Gönczy, Pierre  
Vidal, Marc
Boxem, Mike
van den Heuvel, Sander
Date Issued

2009

Published in
Nature cell biology
Volume

11

Issue

3

Start page

269

End page

77

Subjects

Caenorhabditis-Elegans

•

C-Elegans

•

Genetic Interference

•

Interactome Network

•

Planar Divisions

•

Cleavage Plane

•

Oocyte Cortex

•

Protein

•

Embryos

•

Organization

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPGON  
Available on Infoscience
February 3, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/46416
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