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  4. Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles
 
conference paper

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Hsieh, C.-L.  
•
Grange, R.  
•
Pu, Y.  
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2010
Proceedings of SPIE - The International Society for Optical Engineering
Conference on Biosensing III

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal structures have high second-harmonic generation (SHG) efficiency and provide high contrast in a generally non-structured cell environment. Here, we use barium titanate (BaTiO3) nanoparticles with tetragonal crystal structure as imaging probes. Cytotoxicity tests performed on BaTiO3 nanoparticles with mammalian cells did not result in toxic effects. Specifically we observed no change in the cell metabolism after 24 hours incubation of the cells with high concentration of BaTiO3 nanoparticles. We demonstrate two methods of cell labeling with BaTiO 3 nanoparticles for imaging. One is non-specific labeling via endocytosis of the cells, which results in a great number of the nanoparticles randomly distributed inside the cells. The other is specific labeling via surface functionalization of the nanoparticles with antibodies, which enables us to label specific cell membrane proteins with the nanoparticles. SHG imaging is compatible to two-photon microscopy and the SHG signal from nanoparticles can be easily detected with a standard two-photon confocal microscope. Our work provides the opportunity for long-term, three-dimensional cell tracking with secondharmonic nanoparticles. © 2010 SPIE.

  • Details
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Type
conference paper
DOI
10.1117/12.860595
Scopus ID

2-s2.0-77957849645

Author(s)
Hsieh, C.-L.  
•
Grange, R.  
•
Pu, Y.  
•
Psaltis, D.  
Date Issued

2010

Publisher

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa

Published in
Proceedings of SPIE - The International Society for Optical Engineering
Series title/Series vol.

Proceedings of SPIE-The International Society for Optical Engineering

Volume

7759

Subjects

Second harmonic generation

•

barium titanate

•

nanoparticle

•

microscopy

•

biological cells

•

cytotoxicity

•

endocytosis

•

surface functionalization

•

Generation

•

Probes

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LO  
Event nameEvent placeEvent date
Conference on Biosensing III

San Diego, CA

Aug 01-03, 2010

Available on Infoscience
November 23, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/58105
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