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  4. Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy
 
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research article

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Descloux, A.
•
Grußmayer, K.S.
•
Bostan, E.
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2018
Nature Photonics

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spatial super-resolution. We propose the combination of a novel label-free white light quantitative phase tomography with fluorescence imaging to provide high-speed imaging and spatial super-resolution. The non-iterative phase reconstruction relies on the acquisition of a single image at each z-location and thus enables straightforward 3D phase imaging using a classical microscope. We realized multi-plane imaging using a customized prism for a simultaneous acquisition of 8 planes. This allowed us to not only image live cells in 3D at up to 200 Hz, but also to integrate fluorescence super-resolution optical fluctuation imaging within the same optical instrument. This 4D microscope platform unifies the sensitivity and high temporal resolution of phase tomography with the specificity and high spatial resolution of fluorescence imaging.

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Type
research article
DOI
10.1038/s41566-018-0109-4
Web of Science ID

WOS:000426153800017

Author(s)
Descloux, A.
•
Grußmayer, K.S.
•
Bostan, E.
•
Lukes, T.
•
Bouwens, A.
•
Sharipov, A.
•
Geissbuehler, S.
•
Mahul Mellier, A-L.
•
Lashuel, H. A.  
•
Leutenegger, M.
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Date Issued

2018

Published in
Nature Photonics
Volume

12

Issue

3

Start page

165

End page

172

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LMNN  
LBEN  
Available on Infoscience
July 24, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/139427
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