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  4. Misrouting of tyrosinase with a truncated cytoplasmic tail as a result of the murine platinum (cp) mutation
 
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research article

Misrouting of tyrosinase with a truncated cytoplasmic tail as a result of the murine platinum (cp) mutation

Beermann, F.  
•
Orlow, S. J.
•
Boissy, R. E.
Show more
1995
Exp Eye Res

Mice homozygous for the platinum (cp) allele at the albino locus manifest severe oculocutaneous albinism despite the presence in vitro of tyrosinase activity at 25% wild-type levels. We demonstrate that the cp allele results from an A-->T substitution, changing a lysine residue at position 489 to a termination codon, with truncation of tyrosinase's cytoplasmic tail. In choroidal melanocytes of neonatal mutant mice, tyrosinase activity could be detected in the trans Golgi network, but was absent from melanosomes. Instead, it was detected in vesicles in the cell periphery and dendrites, and on the extracellular surface. In the retinal pigment epithelium, activity was present on the extracellular apical and basolateral surfaces. Our results demonstrate misrouting of a mutant tyrosinase lacking its cytoplasmic tail, providing an explanation for the severe effect of this mutation on ocular and cutaneous pigmentation.

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Type
research article
DOI
10.1016/S0014-4835(05)80053-3
Author(s)
Beermann, F.  
•
Orlow, S. J.
•
Boissy, R. E.
•
Schmidt, A.
•
Boissy, Y. L.
•
Lamoreux, M. L.
Date Issued

1995

Published in
Exp Eye Res
Volume

61

Issue

5

Start page

599

End page

607

Note

Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland.

Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
GR-BEERMANN  
Available on Infoscience
January 22, 2008
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/16432
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