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research article

Open-source microscope add-on for structured illumination microscopy

Hannebelle, Melanie T. M.  
•
Raeth, Esther  
•
Leitao, Samuel M.  
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February 20, 2024
Nature Communications

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to upgrade existing optical microscopes to Structured Illumination super-resolution Microscopes (SIM). The openSIM is an open-hardware system, designed and documented to be easily duplicated by other laboratories, making super-resolution modality accessible to facilitate innovative research. The add-on approach gives a performance improvement for pre-existing lab equipment without the need to build a completely new system.|Researchers developed an open-hardware structured illumination microscopy add-on. This affordable upgrade provides super-resolution capabilities for normal optical microscopes. Detailed instructions enable easy reproduction to help democratize advanced microscopy.

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Type
research article
DOI
10.1038/s41467-024-45567-7
Web of Science ID

WOS:001176075200034

Author(s)
Hannebelle, Melanie T. M.  
•
Raeth, Esther  
•
Leitao, Samuel M.  
•
Lukes, Tomas  
•
Pospisil, Jakub
•
Toniolo, Chiara  
•
Venzin, Olivier François  
•
Chrisnandy, Antonius  
•
Swain, Prabhu Prasad  
•
Ronceray, Nathan  
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Date Issued

2024-02-20

Publisher

Nature Portfolio

Published in
Nature Communications
Volume

15

Issue

1

Article Number

1550

Subjects

Superresolution Microscopy

•

Resolution Limit

•

Live Cells

•

Light

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOB  
LBEN  
LBNI  
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FunderGrant Number

Kommission fr Technologie und Innovation (Commission for Technology and Innovation)

EPFL Open Science Fund

CTI-18330.1

Swiss Commission for Technology and Innovation

ERC-2017-CoG

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Available on Infoscience
April 17, 2024
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/207162
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