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  4. An Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases
 
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research article

An Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases

Ceriotti, L.
•
de Rooij, N. F.  
•
Verpoorte, E.
2002
Analytical Chemistry

A new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the particles of the stationary phase are retained and stabilized. The chips were easily fabricated in poly-(dimethylsiloxane) using deep-reactive-ion-etched silicon masters, and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was packed using a vacuum with 3-μm, octadecylsilanized silica microspheres. The packing was stabilized in the column by a thermal treatment, and its stability and quality were evaluated using in-column indirect fluorescence detection. The effects of voltage on electro-osmotic flow and on efficiency were investigated, and the separation of two neutral compounds was achieved in less than 15 s.

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Type
research article
DOI
10.1021/ac0109467
Author(s)
Ceriotti, L.
•
de Rooij, N. F.  
•
Verpoorte, E.
Date Issued

2002

Published in
Analytical Chemistry
Volume

74

Issue

3

Start page

639

End page

647

Note

280

Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
SAMLAB  
Available on Infoscience
May 12, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/39144
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