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research article

A Versatile, Low-Cost, Multiway Microfluidic Sorter for Droplets, Cells, and Embryos

Utharala, Ramesh
•
Tseng, Qingzong
•
Furlong, Eileen E. M.
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April 24, 2018
Analytical Chemistry

Partitioning and sorting particles, including molecules, cells and organisms, is an essential prerequisite for a diverse range of applications. Here, we describe a very economical microfluidic platform (built from parts costing about U.S. $6800 for a stand-alone system or U.S. $3700, when mounted on an existing fluorescence microscope connected to a computer) to sort droplets, cells and embryos, based on imaging data. Valves operated by a Braille display are used to open and close microfluidic channels, enabling sorting at rates of >2 Hz. Furthermore, we show microfluidic 8-way sorting for the first time, facilitating the simultaneous separation and collection of objects with diverse characteristics/phenotypes. Due to the high flexibility in the size of objects that can be sorted, the low cost, and the many possibilities enabled by imaging technology, we believe that our approach nicely complements existing FACS and μFACS technology.

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Type
research article
DOI
10.1021/acs.analchem.7b04689
Author(s)
Utharala, Ramesh
Tseng, Qingzong
Furlong, Eileen E. M.
Merten, Christoph  
Date Issued

2018-04-24

Published in
Analytical Chemistry
Volume

90

Issue

10

Start page

5982

End page

5988

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LBMM  
Available on Infoscience
February 14, 2020
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/165535
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