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research article

Holographic refractive index detector for application in microchip-based separation systems

Burggraf, N.
•
Krattiger, B.
•
de Mello, A. J.
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1998
The Analyst

A novel detection scheme for capillary electrophoresis on planar glass microchips is presented. The application of a holographic-based refractive index detector to the electrophoretic separation of carbohydrates is described. The microchip device consists of a cyclic (square) separation channel having a circumference of 80 mm, a width of 40 μm and a depth of 10 μm. The volume of the injection scheme is approximately 16 pl. Separation and refractive index detection of a mixture of sucrose, N-acetylglucosamine and raffinose, each at a concentration of 33 mM, was achieved within 17 s of injection. Preliminary results demonstrate the feasibility of using hologram-based refractive index detectors in microchip separation systems. Although the initial detection limits are poor in comparison with alternative techniques, the potential of a universal detector of this kind is clear.

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Type
research article
DOI
10.1039/a801478g
Author(s)
Burggraf, N.
•
Krattiger, B.
•
de Mello, A. J.
•
de Rooij, N. F.  
•
Manz, A.
Date Issued

1998

Published in
The Analyst
Volume

123

Start page

1443

End page

1447

Note

193

Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
SAMLAB  
Available on Infoscience
May 12, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/39105
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