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research article

Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM

Penedo, Marcos  
•
Miyazawa, Keisuke
•
Okano, Naoko
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December 1, 2021
Science Advances

Atomic force microscopy (AFM) is the only technique that allows label-free imaging of nanoscale biomolecular dynamics, playing a crucial role in solving biological questions that cannot be addressed by other major bioimaging tools (fluorescence or electron microscopy). However, such imaging is possible only for systems either extracted from cells or reconstructed on solid substrates. Thus, nanodynamics inside living cells largely remain inaccessible with the current nanoimaging techniques. Here, we overcome this limitation by nanoendoscopy-AFM, where a needle-like nanoprobe is inserted into a living cell, presenting actin fiber three-dimensional (3D) maps, and 2D nanodynamics of the membrane inner scaffold, resulting in undetectable changes in cell viability. Unlike previous AFM methods, the nanoprobe directly accesses the target intracellular components, exploiting all the AFM capabilities, such as high-resolution imaging, nanomechanical mapping, and molecular recognition. These features should greatly expand the range of intracellular structures observable in living cells.

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Type
research article
DOI
10.1126/sciadv.abj4990
Web of Science ID

WOS:000733258700023

Author(s)
Penedo, Marcos  
•
Miyazawa, Keisuke
•
Okano, Naoko
•
Furusho, Hirotoshi
•
Ichikawa, Takehiko
•
Alam, Mohammad Shahidul
•
Miyata, Kazuki
•
Nakamura, Chikashi
•
Fukuma, Takeshi
Date Issued

2021-12-01

Publisher

AMER ASSOC ADVANCEMENT SCIENCE

Published in
Science Advances
Volume

7

Issue

52

Article Number

eabj4990

Subjects

Multidisciplinary Sciences

•

Science & Technology - Other Topics

•

force

•

molecules

•

quantification

•

cytoskeleton

•

nanoneedles

•

microscopy

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBNI  
Available on Infoscience
February 14, 2022
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/185374
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