The effect of the presence of metabolism-induced concentration correlations in the calibration samples on the prediction performance of partial least-squares regression (PLSR) models and mid-infrared spectra from Chinese hamster ovary cell cultures was investigated. Samples collected from batch cultures contained highly correlated metabolite concentrations as a result of metabolic relations. Calibrations based on such samples could only be used to predict concentrations in new samples if a similar correlation structure was present and failed when the new samples were randomly spiked with the analytes. On the other hand, such models were able to predict glucose correctly even if they were based on a spectral range in which glucose does not absorb, provided that the correlations in the calibration and in the new samples were similar. If however, samples from a calibration culture were randomly spiked with the main analytes, much more robust PLSR models resulted. It was possible to predict analyte concentrations in new samples irrespective of whether the correlation structure was maintained or not. Validity of all established models for any given use could be predicted a priori by computing the space inclusion and observer conditions. Predictions from these computations agreed in all cases with the experimental test of model validity. [on SciFinder (R)]