Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities
The procedures for calmodulin-tagged phage and two-filter sandwich assays for the isolation of enzymic activities are presented. In the first method, enzymes are displayed on filamentous phage, and the reaction substrate is anchored on the calmodulin-tagged phage-enzymes using a calmodulin binding peptide. In the second method, enzyme mutants are expressed in bacterial colonies on a porous "master" filter, and the enzymes are released and diffuse to a second reaction filter that closely contacts the master filter. [on SciFinder (R)]
2003
Totowa
1-58829-286-X
313
328
Methods in Molecular Biology
CAN 140:212803 7-1 Enzymes Institute of Pharmaceutical Sciences,Swiss Federal Institute of Technology,Zurich,Switz. Journal 1064-3745 written in English. 37340-95-7 (Biotin ligase) Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities)
OTHER