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  4. Self-assembled melamine microlens arrays for immunofluorescence enhancement
 
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conference paper

Self-assembled melamine microlens arrays for immunofluorescence enhancement

Yang, Hui  
•
Tekin, H. Cumhur
•
Sayah, Abdeljalil  
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2013
Proceedings of the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)
17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)

Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immunocomplex is formed on (3-aminopropyl)triethoxysilane (APTES) microstructures, and then carboxyl-functionalized melamine microparticles with two different sizes are electrostatically self-assembled on the microstructures. Mouse IgG diluted in phosphate buffered saline (PBS) is used as model target antigen and easily detected down to a concentration of 2 ng/mL. We also present a detailed two-dimensional numerical study using the Finite Element Method (FEM).

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Type
conference paper
Author(s)
Yang, Hui  
•
Tekin, H. Cumhur
•
Sayah, Abdeljalil  
•
Gijs, Martinus  
Date Issued

2013

Published in
Proceedings of the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)
Start page

1291

End page

1293

Subjects

Dielectric microparticles

•

Immunoassay

•

Optical detection

•

Photonic nanojet

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LMIS2  
Event nameEvent placeEvent date
17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)

Freiburg, Germany

October 27-31, 2013

Available on Infoscience
November 14, 2013
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/97017
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