Extended-gate field-effect transistor with aptamer functionalisation layer for measuring hormone concentration in biofluids and corresponding fabrication method
The present invention concerns a biosensor (1) for sensing stress hormone cortisol concentration in a biofluid (27). The biosensor (1) comprises: an electrical transistor transducer (3) comprising a transistor gate electrode (17); a sensing electrode element (21, 23) comprising a metal element having a biofluid facing surface, and a graphene layer (23) on the biofluid facing surface of the metal element (21), the sensing electrode element (21, 23) being connected to the transistor gate electrode (17) by an electrical connector (33) to form an extended gate configuration with the transistor gate electrode (17); and a reference electrode (29) configured to be in contact with the biofluid (27), and configured to electrically bias the transistor gate electrode (17) through the biofluid (27). The sensing electrode element (21, 23) is functionalised by at least a layer of aptamers (25) placed indirectly or directly on the graphene layer (23), and configured to catch cortisol hormones in the biofluid (27) to thereby change a surface potential of the sensing electrode element (21, 23).
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Alternative title(s) : (de) Extended-gate-feldeffekttransistor mit aptamer-funktionalisierungsschicht zur messung der hormonkonzentration in bioflüssigkeiten und entsprechendes herstellungsverfahren (fr) Transistor à effet de champ à grille élargie avec couche de fonctionnalisation aptamère pour la mesure de la concentration d'hormones dans les biofluides et procédé de fabrication correspondant
| DOI | Country code | Kind code | Date issued |
EP4009045 | EP | A1 | 2022-06-08 |
US2022170920 | US | A1 | 2022-06-02 |