Preparation of nucleic acid templates for solid phase amplification
The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support
35601246
Also published as: WO2007060456 / EP1957668
Alternative title(s) : (de) Herstellung von nukleinsäurematrizen für die festphasenamplifikation (fr) Preparation de matrices d'acides nucleiques pour leur amplification en phase solide
DOI | Country code | Kind code | Date issued |
EP2918686 | EP | A1 | 2015-09-16 |
EP1957668 | EP | B1 | 2015-04-08 |
WO2007060456 | WO | A8 | 2014-10-30 |
US8168388 | US | B2 | 2012-05-01 |
US2010041561 | US | A1 | 2010-02-18 |
EP1957668 | EP | A1 | 2008-08-20 |
WO2007060456 | WO | A1 | 2007-05-31 |
GB0524069 | GB | D0 | 2006-01-04 |