Production of lactate using crabtree negative organisms expressing recombinant lactate dehydrogenase genes
Crabtree neg. organisms such as Kluyveromyces, Pichia, Hansenula and Candida, are used to make selected org. products such as lactic acid. The organisms are cultured in a first culture medium that includes glucose, under conditions that promote cellular respiration. The organisms are then cultured under a second set of conditions that promote prodn. of the selected org. product. The organisms preferably contain an exogenous lactate dehydrogenase gene. Thus, gene PDC1 encoding pyruvate decarboxylase in Kluyveromyces marxianus was disrupted by site-directed mutagenesis. The LDH gene from Kluyveromyces thermotolerans under control of the Saccharomyces cerevisiae PDC1 promoter was then introduced to this Kluyveromyces marxianus strain by electroporation. The resulting strain was able to produce lactic acid using aerobic conditions for cell growth and anaerobic conditions for lactate prodn. [on SciFinder (R)]
31498177
C12p007-56.
Application: US
2000-574873
Us
99-316490
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Alternative title(s) : (de) Methoden und materialien zur synthese von organischen produkten (fr) Methodes et matieres destinees a la synthese de produits organiques (en) Methods and materials for the synthesis of organic products
Us, (Cargill Dow Polymers LLC, USA)
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CA2374482 | CA | C | 2012-09-18 |
JP4637372 | JP | B2 | 2011-02-23 |
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DE60029440 | DE | T2 | 2007-02-08 |
PT1183385 | PT | E | 2006-11-30 |
DE60029440 | DE | D1 | 2006-08-31 |
AT333509 | AT | T | 2006-08-15 |
EP1183385 | EP | B1 | 2006-07-19 |
AU780135 | AU | B2 | 2005-03-03 |
HU0201204 | HU | A3 | 2004-07-28 |
YU83101 | YU | A | 2004-07-15 |
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HK1045174 | HK | A1 | 2002-11-15 |
CN1367841 | CN | A | 2002-09-04 |
HU0201204 | HU | A2 | 2002-08-28 |
KR20020048910 | KR | A | 2002-06-24 |
CZ20014090 | CZ | A3 | 2002-06-12 |
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EP1183385 | EP | A1 | 2002-03-06 |
NO20015688 | NO | L | 2002-01-21 |
NO20015688 | NO | D0 | 2001-11-21 |
AU5034400 | AU | A | 2000-12-12 |
CA2374482 | CA | A1 | 2000-11-30 |
WO0071738 | WO | A1 | 2000-11-30 |