Previous researches on punching of post-tensioned slabs have shown a number of phenomena significantly influencing their strength and behaviour. However, no general agreement is yet found on a physical theory (either in codes of practice or in design models) suitably describing the influence of prestressing and how should it be accounted on the punching shear behaviour. In this paper, the authors present the results of tests on 15 slabs (3000 x 3000 x 250 mm) tested to failure under different loading conditions. The aim of the tests was to investigate in a separate manner the different actions induced by prestressing on the punching shear strength (in-plane forces, bending moments and bonded tendons). These results are finally investigated on the basis of the physical model of the Critical Shear Crack Theory. The fundamentals of this theory are presented and adapted to post-tensioned slabs, providing a rational explanation of the observed phenomena and measured strengths. (C) 2014 Elsevier Ltd. All rights reserved.