Electrochemical reactions and ionization processes

• Presented at: 18th International Mass Spectrometry Conference, Bremen, GERMANY, Aug 29-Sep 04, 2009
• Published in: European Journal Of Mass Spectrometry (ISSN: 1469-0667), vol. 16, p. 341-349
• IM Publications, 2010

Electrochemical or photo-electrochemical reactions in both electrospray ionization and laser desorption ionization are discussed stressing the role of the electrode reaction in influencing the ionization process. In particular, upon application of a high voltage during electrospray ionization, the emitter includes a working electrode, where redox reactions are observed, such as electro-generation of benzoquinone and metal ions. In contrast, the target plate in laser-induced desorption ionization also acts as a photo-electrode, especially when modified with a mesoporous semiconductor. We illustrate here how these electrochemical reactions can be used for tagging purposes and for oxidative or reductive dissociation reactions.

Keywords: electrospray ionization ; emitters ; Ldi ; titanium dioxide nanoparticle ; semiconductor ; peptide in-source-decay ; cysteine tagging ; proteomics ; Electron Detachment Dissociation ; Microspray Mass-Spectrometry ; Laser-Desorption/Ionization ; Protein Identification ; Desorption-Ionization ; Cysteine Residues ; Disulfide Bonds ; Peptides ; Fragmentation ; Matrix

Reference

• EPFL-CONF-172549
• doi:10.1255/ejms.1081
• View record in Web of Science

Record created on 2011-12-16, modified on 2012-03-21