Selective Breast Cancer Cell Capture, Culture, and Immunocytochemical Analysis Using Self-Assembled Magnetic Bead Patterns in a Microfluidic Chip

• Published in: Langmuir (ISSN: 0743-7463), vol. 26, num. 9, p. 6091–6096
• American Chemical Society, 2010

Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth. A solution of target MCF-7 cells mixed with Jurkat cells was brought inside the microchannel, leading to specific MCF-7 cell capture; the latter were then cultured and evidenced by cell immuno-luminescence.

Keywords: Total Analysis Systems ; Mammalian-Cells ; Device ; Adhesion ; Lysis ; Fibronectin ; Separation ; Protein ; Deposition ; Label

Reference

• EPFL-ARTICLE-149395
• doi:10.1021/la9045572
• View record in Web of Science

Record created on 2010-06-17, modified on 2012-03-28